strain fl2 Search Results


mouse strain carrying floxed exons 2-4 of the meg3 gene (meg3 +/fl(2-4)  (Taconic Biosciences)
90
Taconic Biosciences mouse strain carrying floxed exons 2-4 of the meg3 gene (meg3 +/fl(2-4)
<t>Meg3</t> expression in Meg3Δ(1-4) and Meg3Δ(2-4) mice. (A) There are three Meg3 transcript variants. Variant 3 contains 10 <t>exons.</t> Variants 1 and 2 contain a large alternative exon 10 (10A). Variant 2 has an alternative exon 5 (5A). Quantitative PCRs used to quantify their expression are indicated as numbers above each variant. (B) The SYBR based qRT-PCR was used to determine expression of the Meg3 variants in 11.5 dpc WT embryos (n=7). Gapdh was used as the internal reference gene. ΔCt values were presented as mean+/−SD. The ΔCt values from qPCR #5, 6 and 7 were compared with those from qPCR #1, 2, 3 and 4 using ANOVA multiple comparison tests. * p<0.05, considered to be statistically significant. (C) Relative expression levels of Meg3 variants 1 and 2 compared to all three variants (vs qPCR #1 and 2) or to variant 3 (vs qPCR #3 or 4). The values were calculated using 2-ΔΔCt method as described in the Material and Methods. Student t-test was used to compare values against the reference qPCR. * p<0.05, considered to be statistically significant. (D) Detection of exons in Meg3 transcript was done by qPCR. For example, exon 1 was detected by qPCR #8; exons 1–2 by qPCR #17. Other exons were similarly detected by their respective qPCRs as indicated. (E) Relative expression levels of Meg3 detected with qPCR #3 and 18 in 11.5 dpc Meg3Δ(1-4) embryos compared with their WT littermates. (F) Relative expression levels of Meg3 in 11.5 dpc paternal Meg3Δ(2-4) embryos compared with their WT littermates. (G) Relative expression levels of Meg3 in 11.5 dpc maternal Meg3Δ(2-4) embryos compared with their WT littermates. The levels of Meg3 transcripts detected by qPCR in KO embryos were normalized against the levels in their respective WT littermates, which were designated as 1. A minimum of 6 embryos for each genotype from at least 2 litters were used for gene expression analysis. Student t-test was used to compare values between KO mice and their WT littermates. * p<0.05, considered to be statistically significant. (H) Expression levels of predicted truncated Meg3 transcripts in Meg3Δ(2-4)/+ embryos. The percentage of each transcript in KO embryos compared to their WT littermates were deduced from data presented in (G).
Mouse Strain Carrying Floxed Exons 2 4 Of The Meg3 Gene (Meg3 +/Fl(2 4), supplied by Taconic Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse strain carrying floxed exons 2-4 of the meg3 gene (meg3 +/fl(2-4) - by Bioz Stars, 2026-03
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Meg3 expression in Meg3Δ(1-4) and Meg3Δ(2-4) mice. (A) There are three Meg3 transcript variants. Variant 3 contains 10 exons. Variants 1 and 2 contain a large alternative exon 10 (10A). Variant 2 has an alternative exon 5 (5A). Quantitative PCRs used to quantify their expression are indicated as numbers above each variant. (B) The SYBR based qRT-PCR was used to determine expression of the Meg3 variants in 11.5 dpc WT embryos (n=7). Gapdh was used as the internal reference gene. ΔCt values were presented as mean+/−SD. The ΔCt values from qPCR #5, 6 and 7 were compared with those from qPCR #1, 2, 3 and 4 using ANOVA multiple comparison tests. * p<0.05, considered to be statistically significant. (C) Relative expression levels of Meg3 variants 1 and 2 compared to all three variants (vs qPCR #1 and 2) or to variant 3 (vs qPCR #3 or 4). The values were calculated using 2-ΔΔCt method as described in the Material and Methods. Student t-test was used to compare values against the reference qPCR. * p<0.05, considered to be statistically significant. (D) Detection of exons in Meg3 transcript was done by qPCR. For example, exon 1 was detected by qPCR #8; exons 1–2 by qPCR #17. Other exons were similarly detected by their respective qPCRs as indicated. (E) Relative expression levels of Meg3 detected with qPCR #3 and 18 in 11.5 dpc Meg3Δ(1-4) embryos compared with their WT littermates. (F) Relative expression levels of Meg3 in 11.5 dpc paternal Meg3Δ(2-4) embryos compared with their WT littermates. (G) Relative expression levels of Meg3 in 11.5 dpc maternal Meg3Δ(2-4) embryos compared with their WT littermates. The levels of Meg3 transcripts detected by qPCR in KO embryos were normalized against the levels in their respective WT littermates, which were designated as 1. A minimum of 6 embryos for each genotype from at least 2 litters were used for gene expression analysis. Student t-test was used to compare values between KO mice and their WT littermates. * p<0.05, considered to be statistically significant. (H) Expression levels of predicted truncated Meg3 transcripts in Meg3Δ(2-4)/+ embryos. The percentage of each transcript in KO embryos compared to their WT littermates were deduced from data presented in (G).

Journal: Developmental biology

Article Title: Meg3-DMR, not the Meg3 gene, regulates imprinting of the Dlk1-Dio3 locus

doi: 10.1016/j.ydbio.2019.07.005

Figure Lengend Snippet: Meg3 expression in Meg3Δ(1-4) and Meg3Δ(2-4) mice. (A) There are three Meg3 transcript variants. Variant 3 contains 10 exons. Variants 1 and 2 contain a large alternative exon 10 (10A). Variant 2 has an alternative exon 5 (5A). Quantitative PCRs used to quantify their expression are indicated as numbers above each variant. (B) The SYBR based qRT-PCR was used to determine expression of the Meg3 variants in 11.5 dpc WT embryos (n=7). Gapdh was used as the internal reference gene. ΔCt values were presented as mean+/−SD. The ΔCt values from qPCR #5, 6 and 7 were compared with those from qPCR #1, 2, 3 and 4 using ANOVA multiple comparison tests. * p<0.05, considered to be statistically significant. (C) Relative expression levels of Meg3 variants 1 and 2 compared to all three variants (vs qPCR #1 and 2) or to variant 3 (vs qPCR #3 or 4). The values were calculated using 2-ΔΔCt method as described in the Material and Methods. Student t-test was used to compare values against the reference qPCR. * p<0.05, considered to be statistically significant. (D) Detection of exons in Meg3 transcript was done by qPCR. For example, exon 1 was detected by qPCR #8; exons 1–2 by qPCR #17. Other exons were similarly detected by their respective qPCRs as indicated. (E) Relative expression levels of Meg3 detected with qPCR #3 and 18 in 11.5 dpc Meg3Δ(1-4) embryos compared with their WT littermates. (F) Relative expression levels of Meg3 in 11.5 dpc paternal Meg3Δ(2-4) embryos compared with their WT littermates. (G) Relative expression levels of Meg3 in 11.5 dpc maternal Meg3Δ(2-4) embryos compared with their WT littermates. The levels of Meg3 transcripts detected by qPCR in KO embryos were normalized against the levels in their respective WT littermates, which were designated as 1. A minimum of 6 embryos for each genotype from at least 2 litters were used for gene expression analysis. Student t-test was used to compare values between KO mice and their WT littermates. * p<0.05, considered to be statistically significant. (H) Expression levels of predicted truncated Meg3 transcripts in Meg3Δ(2-4)/+ embryos. The percentage of each transcript in KO embryos compared to their WT littermates were deduced from data presented in (G).

Article Snippet: To obtain this strain, a mouse strain carrying floxed exons 2-4 of the Meg3 gene (Meg3 +/fl(2-4) ) was created using services from Taconic (TaconicArtemis, Cologne, Germany).

Techniques: Expressing, Variant Assay, Quantitative RT-PCR, Comparison, Gene Expression